Advanced In-vivo Imaging and Manipulation

Description

 

We combine advanced optics/software/microfabrication/optical instrumentation to deliver in-vivo microscopy systems.

Light has influenced across multi-disciplines of sciences, engineering, energy and medicine. Light microscopy has the ability to see the very small to the very big (from nanoscale to macroscale), which illustrate the marvels and power of light. The appeal of microscopy, much like astronomy, augments the limitations of human vision and allow us to see the seemingly invisible microscopic world.
 
Portable Microscopy
For centuries, lenses are traditionally made with complex machinery because the need for fine grinding and re-flow moulding techniques to create smooth lens surface. In refractive lenses, the focal length is intricately linked to the curvature of the lenses. We harvest solid polymer lenses from hanging liquid droplets which are then incorporated into a wearable microscope platform that is use to look at macroscopic to microscopic objects in the field. We also make use of computational techniques to increase the performance of these lenses.
 
Digital Holographic Microscopy
We develop an automated quantitative microscope platform based on holography that captures structural information of live biological cells without fluorescence labelling.
 
Surface wave Microscopy (NEW)
We plan to develop high resolution imaging system based on surface waves (total internal reflection) for probing nanoscale biological dynamic.
 
Intravital Microscopy
Whilst imaging cellular activities in thin samples are readily perform in laboratories, imaging cellular activities in an entire intact whole organ with light is a grand challenge in optical microscopy. The advent of laser scanning microscopy (confocal, multiphoton) coupled with specific fluorescent proteins are indispensable for visualising many cellular level activities that help to decipher the fundamentals of many biological processes. We have developed a flexible video-rate two photon microscopy system capable of imaging microvasculature flow in living mice.
 

Goals

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Requirements

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Background Literature

- "Automated Fourier space recognition filtering for off-axis digital holographic microscopy", Biomed. Opt. Express 7(8), 3111-3123 (2016)

-“Intravital Microscopic Interrogation of Peripheral Taste Sensation”, Scientific Reports 5 8661 (2015)

-"Hollow Bessel-like beam as an optical guide for a stream of microscopic particles," Opt. Express 21, 30492  (2013)

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Keywords

Intravital Microscopy, Optical Trapping, Digital Holographic Microscopy, Force spectroscopy

Updated:  8 September 2015/Responsible Officer:  Dean, CECS/Page Contact:  CECS Marketing