Dr Steve Lee

Senior Lecturer

Research Area:

PhD (Optics), B.Eng (EE)

Craig Building (35A), R204

steve.lee@anu.edu.au

+61 2 6125 6058

+61 2 6125 5476

» read more about Dr Steve Lee

Bio
Research projects
Student projects
Publications
Students
Service
Courses

Steve Lee completed his undergraduate training in Engineering at the Nanyang Technological University (Singapore) and a PhD in Optics at Optical Trapping Group at the University of St Andrews (UK) with Prof Kishan Dholakia. During his PhD, he developed new optical manipulation technologies for biological and nanoparticles. He performed the first conclusive nonlinear light measurements on artifical kerr medium and built a multimodality bioworkstation. In 2010, he left the UK to pursue a 2-year postdoctoral training at Wellman Photomedicine/Harvard Medical School (USA) with Prof S H Andy Yun, where he developed adaptive intravital endomicroscopy for live small animal imaging. That is followed by a short stint as a Vice Chancellor Fellow at UNSW on nanowire optical manipulation. In 2013, he started his own research group (Applied Optics Lab) at ANU Research School of Engineering that focus on developing novel optical techniques and instruments to harness light for application in biomedicine and nanotechnology, where he recently won the 2014 ANSTO Eureka Prize for developing low cost elastomer optics. In 2014, he joins ARC Centre of Excellence for Advanced Molecular Imaging as an Associated Investigator to develop the next generation of intravital microscopy for in-vivo imaging. In 2015, he receive an ARC Discovery Early Career Research Award (DECRA). In 2016, he joins ARC Centre of Excellence for Nanoscale Biophotonics as an Associated Investigator.

Lead

Current student projects

Principal investigator

Advanced In-vivo Imaging and Manipulation

Supervisor

Recent publications

† Corresponding * Equal contribution

News Article

Droplet lens work highlighted by Jon Cartwright,"Technology: Smartphone science" Nature 531,669–671 (2016) link
W.M Lee †, "Pocket Pathology",The Pathologist - Texere Publishing 314, 39-41 (2014) - link
W.M. Lee †, T Kamal , “A droplet approach to lens making”, The Australian Optical Society 28 30-31 (2014) - link

Journal

Y X Li * , V Gautam, A Brustle, I A Cockburn, V R Daria, C Gillespie, K Gaus, C Alt, and W M Lee †*, " Flexible polygon-mirror based laser scanning microscope platform for multiphoton in-vivo imaging" Journal of Biophotonics (2017) 10.1002/jbio.201600289 (Early View) (Preprint)
T Kamal, R Watkins, Z Cen, J Rubinstein, G Kong and W.M.Lee† “ Design and fabrication of a passive droplet dispenser for portable high resolution imaging system" Scientific Reports 7 41482 (2017) link
X F He, C V Nguyen, M Pratap, Y J Zheng, Y Wang, D. R. Nisbet, R J Williams, M Rug, A G. Maier, W M Lee† "Automated Fourier space recognition filtering for off-axis digital holographic microscopy", Biomed. Opt. Express 7(8), 3111-3123 (2016) link
Wenguo Zhu*, Niko Eckerskorn, Avinash Upadhya , Li Li, Andrei V. Rode† and W M Lee*† "Dynamic axial control over optically levitating particles in air with an electrically-tunable variable-focus lens" Biomedical Optics Express 7(7), 2902-2911 (2016) link
W.M. Lee †, “Miniature Droplet Lenses for mobile microscopy”, SPIE Newsroom 10.1117/2.1201505.005972 (2015) link
T Kamal, X F He, W.M Lee †, "Reinventing Pocket Microscopy",InFocus (Proceedings of the Royal Microscopical Society) 37, 41- 43 (2015)pdf,link
M Choi, W.M. Lee, S H Yun, “Intravital Microscopic Interrogation of Peripheral Taste Sensation”, Scientific Reports 5 8661 (2015) link
W.M. Lee †, “Frugal microscopy: Inspiring tomorrow’s interdisciplinary scientist “ InFocus (Proceedings of the Royal Microscopical Society) 36, 24-25 (2014) Letter to Editor pdf
W.M. Lee †, A. Upadhya, P.J. Reece, T G Phan, "Fabricating Low Cost and High Performance Elastomer Lenses using Hanging Droplets", Biomedical Optics Express 5 1626-1635 (2014) link
L Li, W.M.Lee †, X.S Xie, W.Z Krolikowski, A.V. Rode, J Y Zhou, "Shaping Self-imaging Bottle Beams with Modified Quasi-Bessel Beams", Opt. Lett. 39, 2278-2281 (2014). pdf
Niko Eckerskorn, Li Li, Richard A. Kirian, Jochen Küpper, Daniel P. DePonte, Wieslaw Krolikowski, W. M. Lee, Henry N. Chapman, and Andrei V. Rode, "Hollow Bessel-like beam as an optical guide for a stream of microscopic particles," Opt. Express 21, 30492-30499 (2013) link
F Wang, W Toe, W.M Lee et al “Resolving Stable Axial Trapping Points of Nanowires in an Optical Tweezers Using Photoluminescence Mapping" Nanoletters 13 1185-1191 (2013) pdf

Conference

T J McMenamin, W M Lee† “A Compact Multi-Trap Optical Tweezer System based on CD-ROM Technologies” SPIE Nanoscience and Engineering (2017) Submitted
Y X Li, J D A Bermudez, W Zhong, R Mahony, W.M.Lee † “Tracking of Non-Fluorescence Blood Cells with High Speed Multiphoton Intravital Microscopy”, IEEE International Symposium on Biomedical Imaging (2017) Accepted
X F He, K Gaus, W M Lee † “Label-free dynamic volumetric imaging of deforming giant unilamellar vesicles under micro-flows”, OSA BioOptics Design (2017) Accepted
Y X Li,, H M Huang, G Burgio, W Heath and, Woei Ming Lee † , “Dynamic control over field of view in polygon mirror-based laser scanning multiphoton microscope” OSA BioOptics Design (2017) Accepted
T Kamal, J Rubinstein, R Watkins, Z Cen, G Kong and W.M.Lee † “Thimble microscope system” SPIE BioPhotonics Australasia 10013, 1001322-1001322-5 (2016)
T Kamal, R Watkins, Z Cen, and W.M.Lee † “Direct fabrication of silicone lenses with 3D printed parts” SPIE BioPhotonics Australasia, 1001336-1001336-6 (2016)
X F He, Y Zheng, W. M Lee †, “Dynamic holographic microscopy interface”, SPIE BioPhotonics Australasia 10013, 1001332-1001332-8 (2016)
X He, C V Nguyen, M Pratap, Y Zheng, Y Wang, D R. Nisbet, A. G. Maier, W.M.Lee † “Adaptive spatial filtering for off-axis digital holographic microscopy based on region-recognition approach with iterative thresholding” SPIE BioPhotonics Australasia 10013, 1001329-1001329-7 (2016)
Y X Li, W.M.Lee † “PScan1.0 : Flexible software framework for polygon based multiphoton microscopy “SPIE BioPhotonics Australasia 10013, 1001333-1001333-6 (2016)
Y X Li, A Brustle, V Gautam, I Cockburn, V R Daria, C Gillespie, K Gaus and W.M.Lee † “ High speed multiphoton imaging” SPIE BioPhotonics Australasia 10013, 100130K (2016)
W Zhu, N Eckerskorn, W M Lee † and A V. Rode" Dynamic axial control over optically levitating particles in air with an electrically-tunable variable-focus lens" ICONN (2016)
R. Fleddermann, W. M. Lee †, K Huang, G. Campbell , P. K. Lam , J. H. Chow , D. E. McClelland, "Compact 20 kHz delay line with cascading multi-level phase plate for low coherence interferometry" ANZCORP(2015)
X.F. He, A. Maier, W.M. Lee† "Mapping the progression of malaria infected erythrocytes with holographic microscopy" Conference on Lasers and Electro-Optics/Pacific Rim, 27P_73
W. M Lee†, D Wright, R Watkins, Zi Cen, "Integrated elastic microscope device" Proceedings of SPIE-BIOs Optical Diagnostics and Sensing XV: Toward Point-of-Care Diagnostics 93320 (2015)
M Choi, W.M. Lee, S H Yun, “Intravital Microscopic Interrogation of Peripheral Taste Sensation”, Annual Meeting of the Society for Neuroscience(2014)
W. M. Lee†, “Imaging Cells in Living Animals with two photon in-vivo aberration free endomicroscopy” Australian Biomedical Engineering Conference 2014
(Invited) W. M. Lee†, "High performance and low cost elastomer optics," in Classical Optics 2014, OSA Technical Digest (online) (Optical Society of America, 2014), OTh4B.1.
W. M. Lee†, "Stick-On Microscope for Smartphones” Proceedings of SPIE-BIOs Optical Diagnostics and Sensing XIV: Toward Point-of-Care Diagnostics 8951-16 (2014)
W.M. Lee†, "Microscopy On the Move”, SPIE Digital Photography X 9023-9 (2014)
Li Li, N Eckerskorn, R A. Kirian J Küpper, D P. DePonte, Krolikowski, W.M. Lee, H N. Chapman and A V. Rode “Quasi- Bessel hollow beam as optical guide for micro-particles” SPIE Optical Trapping and Manipulation (2013)